SrasV1, Main, Exploration, bibRecord, 004D53

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Identifieur interne : 004D53 ( Main/Exploration ); précédent : 004D52; suivant : 004D54

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Auteurs : K. Hattermann [Allemagne] ; M. A. Müller [Allemagne] ; A. Nitsche [Allemagne] ; S. Wendt [Allemagne] ; O. Donoso Mantke [Allemagne] ; M. Niedrig [Allemagne]

Source :

Archives of virology [ 0304-8608 ] ; 2005.

RBID : Pascal:05-0484477

Descripteurs français

KwdFr :
- Animaux, Carboxypeptidases (génétique), Carboxypeptidases (métabolisme), Cellules Vero, Cellules eucaryotes (virologie), Chats, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, RT-PCR, Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Souris, Suidae, Virus du SRAS (génétique), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie).
MESH :
- génétique : Carboxypeptidases, Récepteurs viraux, Virus du SRAS.
- métabolisme : Carboxypeptidases, Récepteurs viraux.
- pathogénicité : Virus du SRAS.
- physiologie : Virus du SRAS.
- virologie : Cellules eucaryotes.
Pascal (Inist)
- Animaux, Cellules Vero, Chats, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, RT-PCR, Souris, Suidae, Virus syndrome respiratoire aigu sévère, Sensibilité, Lignée cellulaire, Pouvoir infectant.

English descriptors

KwdEn :
- Animals, Carboxypeptidases (genetics), Carboxypeptidases (metabolism), Cats, Cell Line, Cell line, Chlorocebus aethiops, Eukaryotic Cells (virology), Humans, Infectivity, Mice, Peptidyl-Dipeptidase A, Receptors, Virus (genetics), Receptors, Virus (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Sensitivity, Severe acute respiratory syndrome virus, Swine, Vero Cells.
MESH :
- chemical , genetics : Carboxypeptidases, Receptors, Virus.
- chemical , metabolism : Carboxypeptidases, Receptors, Virus.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- physiology : SARS Virus.
- virology : Eukaryotic Cells.
- Animals, Cats, Cell Line, Chlorocebus aethiops, Humans, Mice, Peptidyl-Dipeptidase A, Reverse Transcriptase Polymerase Chain Reaction, Swine, Vero Cells.

Abstract

In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS-Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.

Affiliations:

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Carboxypeptidases (genetics)</term>
<term>Carboxypeptidases (metabolism)</term>
<term>Cats</term>
<term>Cell Line</term>
<term>Cell line</term>
<term>Chlorocebus aethiops</term>
<term>Eukaryotic Cells (virology)</term>
<term>Humans</term>
<term>Infectivity</term>
<term>Mice</term>
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<term>Receptors, Virus (genetics)</term>
<term>Receptors, Virus (metabolism)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (pathogenicity)</term>
<term>SARS Virus (physiology)</term>
<term>Sensitivity</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Swine</term>
<term>Vero Cells</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Carboxypeptidases (génétique)</term>
<term>Carboxypeptidases (métabolisme)</term>
<term>Cellules Vero</term>
<term>Cellules eucaryotes (virologie)</term>
<term>Chats</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Peptidyl-Dipeptidase A</term>
<term>RT-PCR</term>
<term>Récepteurs viraux (génétique)</term>
<term>Récepteurs viraux (métabolisme)</term>
<term>Souris</term>
<term>Suidae</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (pathogénicité)</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Receptors, Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxypeptidases</term>
<term>Receptors, Virus</term>
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<term>Récepteurs viraux</term>
<term>Virus du SRAS</term>
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<term>Récepteurs viraux</term>
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<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
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<term>Mice</term>
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<term>Peptidyl-Dipeptidase A</term>
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<term>Suidae</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Sensibilité</term>
<term>Lignée cellulaire</term>
<term>Pouvoir infectant</term>
</keywords>
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<front><div type="abstract" xml:lang="en">In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS-Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.</div>
</front>
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Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

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English descriptors

Abstract

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Le document en format XML

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Pour mettre un lien sur cette page dans le réseau Wicri

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